I attempted the crosslinking method using glutaraldehyde, which resulted in pellet formation that is challenging to dissolve, possibly due to excessive crosslinking. I'm now seeking an alternative approach.
I've tried various 2.5% concentrations of glutaraldehyde, but all of them yield pellets that are somewhat challenging to dissolve. that's why I need an alternative approach. I performed cross-linking for 2 hours initially, and then I tried extending the time to 6 hours as well as reducing it to 1 hour. However, I didn't observe any significant changes.
( It's worth noting that I purified my cellulase protein using ammonium sulfate precipitation before starting the cross-linking process. Is it appropriate to use ammonium sulfate precipitation-purified protein for this crosslinking purpose?:
In my laboratory, two students attempted to work with xylanase and exo-cellulase, and both of them achieved success on their very first try. They didn't encounter any significant difficulties, not even when it came to dissolution.
I'm feeling unsure about what steps to take, and time is slipping away quickly. I seem to be stuck in this situation.