Abhi Abhi

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Questions related from Abhi Abhi

I conducted Carbonic Anhydrase activity assay using p-nitrophenylacetate. However,I am obtaining inaccurate absorption results. what should i do ?

I am doing a carbonic anhydrase activity assay using p-nitrophenyl acetate. reaction conditions: 37 °C for 10 minutes Blank: 40ul substrate + 160 ul 50mM Tris buffer pH 7.4 crude: 40 ul...

25 March 2024 1,894 1 View

Please provide a more accessible and cost-effective immobilization technique for the Cellulase enzyme.?

I attempted the crosslinking method using glutaraldehyde, which resulted in pellet formation that is challenging to dissolve, possibly due to excessive crosslinking. I'm now seeking an alternative...

27 October 2023 7,005 2 View

Suggest a simpler and less problematic immobilization technique for cellulase (I've had difficulties with the crosslinking (CLEA) method) ?

Right now, I'm using the Cross-linked enzyme aggregates (CLEA) method for immobilizing cellulase enzyme. While there are several other methods to choose from, I decided to go with CLEA for...

09 August 2023 9,181 0 View

How to dissolve cross-linking enzyme. I have problem with dissolving.?

I performed crosslinking using a 10 mg pellet and 5 ml of diluted 2.5% glutaraldehyde for a period of 2 hours. Upon attempting to dissolve the crosslinked enzyme for activity assay, I encountered...

28 April 2023 6,034 3 View

Please provide a more accessible and cost-effective immobilization technique for the Cellulase enzyme?

I attempted the crosslinking method using glutaraldehyde, which resulted in pellet formation that is challenging to dissolve, possibly due to excessive crosslinking. I'm now seeking an alternative...

01 January 1970 7,254 2 View