Hi Mirian,

actually, there are many ways to build Arlequin files. However, it would be useful to know what kind of genetic data you have. A general recommendation I could give you is to try to format your data in the GenAlex (http://biology-assets.anu.edu.au/GenAlEx/Welcome.html) which is way easier to visualize your data. After generating your Arlequin data file, when you load it in Arlequin, never forget to check the structure settings tab. If you want to test differences between groups of populations, you have to set it there.

If you have any further question.

Just let me know

Good Luck!

Hi!

As Wilson said, there's many ways.

For generating the input files for Arlequin you can take a look at the manual:

http://cmpg.unibe.ch/software/arlequin35/man/Arlequin35.pdf

In pages 44-50 you have several examples of input files depending on your data. You can take one of the example files and just modify it with your data:

http://cmpg.unibe.ch/software/arlequin35/Arl35Downloads.html

Be careful with the "Profile" lines! (in page 29 of the manual you have all the information you need)

[Profile]

NbSamples=10 ## in your case this is 6

GenotypicData=0 ## it depends on your type of data

LocusSeparator=NONE ## page 29 of the manual

DataType=RFLP

CompDistMatrix=0

[Data]

[[Samples]]

SampleName="POPULATION NAME"

SampleSize= 14 ## or 12 or 13

SampleData= {

... }

Best!

Thanks Wilson and Noemi, your guidelines were really helpful. I can now preper arliquin input file of various forms and run my analysis in several ways. Once again, thank you.