Please help me How can I free access to UPtodate?

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How can I check primer pair sequence before order?

Before ordering primer pair sequence , how can I check them? what is the accepted range of different primers properties , e.g : self complementary , haipin ......? if GC % less than 40 % for the...

09 October 2017 3,579 0 View

The sequence written in Genbank file as sense strand or both strand?

are The sequences of any gene in ncbi database that given in genbank file as DNA written as both strand ? or just one of them so how can we design primer F and R primer?

09 October 2017 2,603 4 View

Primer design: positive single strand viruses and also negative single strand virus?

If the virus is positive single strand ( i.e make as mRNA ) ,I will put the genomic as an input for primer3 then click on right primer and left primer: then it gives us the forward and reverse...

09 October 2017 8,843 1 View

How can I know the recognition site of Restriction enzyme?

I want to replace primer pair for my target gene with other primer pair for the same target but in different location , it target smaller range of sequence than the previous , so my question is...

08 September 2017 2,343 0 View

I have a question about Detection of Brucella by PCR : no result on gel of product that is positive by real time pcr?

I have made extraction of brucella DNA from vagnal swab using QIAMP DNA mini kit qiagen then I made real time PCR USING applied biosystem kit for detection brucella sp. the result is strong...

08 September 2017 1,388 3 View

I have a problem with PCR, why I have pcr product size that is not expected?

why I have different pcr product size , although the same primer pairs sequence were used in many previous study and result the same pcr product size, but by me I have a different pcr product...

08 September 2017 5,887 8 View

Primer design for RNA virus detection by PCR?

Hi I want to design PCR primers for Infectious Bronchitis (IB) , Can any expert tell me If the genome sequences that I will use it as input sequence for primer3 : CDS of the genome to be as cDNA ?

02 March 2017 3,903 2 View

Can I make PCR for Brucella spp. without bacterial culturing step?

I make DNA extraction of vaginal swab brucella then Amplification using specfic primers for Brucella meltinesis but the result was no pcr products no any band when I search I was found I MUST...

01 February 2017 6,112 3 View

Which Scopus Journal provides the most affordable fees?

"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....

10 August 2024 8,621 1 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Could you please suggest methods to compare free protein and immobolized protein binding properties?

I have an antibody binding generic protein and I need to compare its activity in a free and immobolized form. I understand that there are a number of methods to determine Kd value of a free...

05 August 2024 5,311 0 View

Where can I find free research instruments for Nursing?

for eg. transition shock scale

01 August 2024 5,998 0 View

Why do open and free science in a world where science is not open and free?

Because I have realized that the world tends more and more to do open and free science and there is a trend more and more to choose free databases, free tools and open access platforms.

01 August 2024 10,046 1 View

My cfDNA extraction protocol using silica beads works with plasma collected in COD SAGM bags but not with plasma collected in K2 EDTA tubes. Why so?

I am currently performing cell-free DNA (cfDNA) extraction from plasma using an in-house developed kit that employs silica-coated magnetic beads. My protocol works effectively with plasma obtained...

28 July 2024 3,559 0 View

Do you know of any online international conferences that offer free discussions?

Do you know of any online international conferences that offer free discussions? I am looking for examples in the field of molecular ecology and DIY biology.

28 July 2024 6,501 0 View

Why binding energy is in positive number after performing mmpbsa?

I performed mmpbsa of protein-protein complex simulation on gromacs. My binding free energy is +166781. This complex is stable when experimentally produced in wet lab. In index.ndx I grouped...

19 July 2024 4,120 5 View

How to download free version of BiofilmQ ?

I would like to use biofilmQ to analyse confocal laser image, how can do?

16 July 2024 7,628 2 View

Qiagen circulating nucleic acid kit poor yield?

Hi, Attempted to extract cfDNA from a 4 mL plasma sample, with poor yield. Went through the manual in the section regarding poor yield, and feel that this does not apply. Some potential...

14 July 2024 7,475 1 View