How can I check primer pair sequence before order?

Before ordering primer pair sequence , how can I check them? what is the accepted range of different primers properties , e.g : self complementary , haipin ......? if GC % less than 40 % for the...

09 October 2017 3,549 0 View

The sequence written in Genbank file as sense strand or both strand?

09 October 2017 2,575 4 View

Primer design: positive single strand viruses and also negative single strand virus?

09 October 2017 8,810 1 View

How can I know the recognition site of Restriction enzyme?

08 September 2017 2,314 0 View

I have a question about Detection of Brucella by PCR : no result on gel of product that is positive by real time pcr?

08 September 2017 1,344 3 View

I have a problem with PCR, why I have pcr product size that is not expected?

08 September 2017 5,835 8 View

Primer design for RNA virus detection by PCR?

02 March 2017 3,877 2 View

Can I make PCR for Brucella spp. without bacterial culturing step?

01 February 2017 6,080 3 View

Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

Is there an open source free software for text mining and sentiment analysis?

I am looking for a free software for text mining and sentiment analysis for my research on customer review mining (it involves calculating polarity of attributes,opinion oriented information...

25 February 2021 1,920 6 View

Which are the journals for free paper publication?

A bunch of flower for everybody. I would like to ask about the list of journals which we can publish our paper for free. Thanks in advance. Sincerely Walid

25 February 2021 9,790 3 View

Suggestions for occupational stress surveys?

I am currently writing a masters thesis on reasons for occupational stress in the early childhood classroom, and want to include a short survey assessing participants work-related stress levels. I...

24 February 2021 8,678 2 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Hello, does any one know a free medical text dataset?

a free multi label clinical text dataset , containing text as entry and several labels as classes. Best regards

22 February 2021 5,988 2 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View

Can anyone recommend which is the best free questionnaire software please?

Hello, I am looking to send out links to an online survey. My previous research project used a paid for survey software. I am now looking for something that's free but straight forward to...

19 February 2021 5,641 3 View

How to obtain free lipids starting from concentration over the CMC?

I need to work with free lipids at increasing concentration (50%, 60% of the CMC ect.). I'm now working with POPC and POPG. To reach these concentrations I have to start from concentration over...

17 February 2021 1,676 3 View