Israa Hassona

9 Questions 8 Answers 0 Followers

Questions related from Israa Hassona

Please help me How can I free access to UPtodate?

can anyone help me to access to uptodate site? I need to get some medical information from uptodate site but the problem is that need payment for getting password please help thank you in advanced

03 March 2018 9,671 4 View

How can I check primer pair sequence before order?

Before ordering primer pair sequence , how can I check them? what is the accepted range of different primers properties , e.g : self complementary , haipin ......? if GC % less than 40 % for the...

10 October 2017 3,560 0 View

Primer design: positive single strand viruses and also negative single strand virus?

If the virus is positive single strand ( i.e make as mRNA ) ,I will put the genomic as an input for primer3 then click on right primer and left primer: then it gives us the forward and reverse...

10 October 2017 8,821 1 View

The sequence written in Genbank file as sense strand or both strand?

are The sequences of any gene in ncbi database that given in genbank file as DNA written as both strand ? or just one of them so how can we design primer F and R primer?

10 October 2017 2,583 4 View

I have a problem with PCR, why I have pcr product size that is not expected?

why I have different pcr product size , although the same primer pairs sequence were used in many previous study and result the same pcr product size, but by me I have a different pcr product...

09 September 2017 5,848 8 View

How can I know the recognition site of Restriction enzyme?

I want to replace primer pair for my target gene with other primer pair for the same target but in different location , it target smaller range of sequence than the previous , so my question is...

09 September 2017 2,324 0 View

I have a question about Detection of Brucella by PCR : no result on gel of product that is positive by real time pcr?

I have made extraction of brucella DNA from vagnal swab using QIAMP DNA mini kit qiagen then I made real time PCR USING applied biosystem kit for detection brucella sp. the result is strong...

09 September 2017 1,360 3 View

Primer design for RNA virus detection by PCR?

Hi I want to design PCR primers for Infectious Bronchitis (IB) , Can any expert tell me If the genome sequences that I will use it as input sequence for primer3 : CDS of the genome to be as cDNA ?

03 March 2017 3,885 2 View

Can I make PCR for Brucella spp. without bacterial culturing step?

I make DNA extraction of vaginal swab brucella then Amplification using specfic primers for Brucella meltinesis but the result was no pcr products no any band when I search I was found I MUST...

02 February 2017 6,092 3 View