Dear Prof. Mauricio Jerez,

Good day,

POPGENE (population genetic analysis) has to be used to estimate the genetic structure parameters for each marker total loci, polymorphism loci and percentage, Shannon's Information Index (I), gene diversity (H) Nei's, Effective number of alleles (Ne) and Observed number of alleles (Na). Analysis of molecular variance (AMOVA) must be estimated using GenAlEx.

Dear Dr Jerez, I think NCBI includes many softwares should work for this purpose . check it to do.

Dear Dr. Jerez,

Perhaps you are familiar with the term DNA Barcoding. This method is not an alternative for classical taxonomy but rather a addon to increase the stringency of taxonomic study. With the advent and use of this technology it has been seen that many plant/ animal species although have tremendous physiological similarity, but they represent two far off families genetically. And the exact opposite phenomenon has also been seen. Huge amount of work in this field is going on across the globe, which you can find out online.

Thank you very much for your anwers. I agree with the argument of @Nirjhar. I would like to have a very basic view from the sampling methods used for applying these techniques in plants and how to take into account the possible pitfalls leading to missidentification from DNA barcoding.