Please anyone have protocol stability testing heat, temperature and PH for actinobacterial spores?

Sitaram Sapkota @Sitaram-Sapkota

22 April 2020 0 3K Report

please, anyone, have protocol stability testing heat, temperature, and PH for actinobacterial spores

Badges
Science topic

More Sitaram Sapkota's questions See All

Is Short Form SF12 , HRQOL is free for use?

or do we need to take permission or it's free.

10 January 2024 7,829 0 View

What are the relevant statistical tests to analyze multiple categorical outcome variables with predicotr variables?

Please suggest me relevant statistical tests to analyze relationship of multiple categorical outcome variables at a time with predictor variables.

14 October 2023 3,606 1 View

How can make rat brain hard for fixing in microtome immediately after extraction keeping cells alive for primary culture?

Is there any specific protocol to extract arcuate nucleus from rat hypothalamus using microtome, keeping cells alive for primary culture?

23 February 2023 1,556 0 View

How to simulate crack growth in Abaqus using Paris law for nitinol (super elasticity) material??

I am trying to simulate crack growth in ABAQUS using Paris law. But I am getting super elasticity convergence error. I am using the direct cycle step. I can easily simulate linear materials. But I...

07 February 2022 4,376 2 View

How do you prepare a high osmolarity 4% PFA for fixing 300 um brain slices?

I am trying to use 300 um hippocampal sections for immunohistochemistry but the antibodies have not penetrated deep enough (

25 October 2021 1,325 0 View

How to get a high virus titre of Chikungunya virus (CHIKV) propagated on Vero cells?

I propagated CHIKV on C6/36 before and harvested on different days of infections, though the results of immunofluorescence assay are highly positive for infection, I cannot get good plaque...

09 August 2021 4,217 1 View

Hall effect measurement of CIS films?

What would be the best metal contact for Copper Indium Selenide (CIS) to measure the Hall effect? Film thickness is about 2 micron and it is copper poor (Cu/In = 0.9).

14 March 2021 3,339 2 View

[SAP2000 related] I wonder why there is no any effect in the analysis result when I change the different parameters while modeling a frame structure?

(i) I have modeled a simple 2D frame structure (2 columns and 1 beam). I would like to see the variation in the result by varying the different parameters such as, sizes of confinement bars,...

06 October 2020 9,704 5 View

If a word file is shared with a person via Google Docs, can the recipient have access to the other files?

We can share and edit 'word' files online via Google Docs. It is an easy and convenient way of creating, sharing and editing the same document online between the concerned users. However, my...

22 September 2020 843 3 View

If you are working with new cell line, what could be the best way to optimize transfection protocol using the Neon® Transfection System ?

I am working with new fish cell lines especially from cold-water fishes, so how can we choose the best program and process to get cells with higher viability and transfection efficiency?...

16 September 2020 9,747 2 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

How to fix errors in my heat transfer steel structure with reinforced concrete slab model Abaqus?

I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...

07 August 2024 1,028 0 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

How to heat polymer for about 130° without oil bath ?

is there any other way to heat a polymer evenly other than oil bath if so what are those and how to use it

06 August 2024 947 1 View

What should I do with parameters that are not relate to my simulation in MyLake model?

I want to Estimate surface heat fluxes using MyLake, but I don't have all the initial values in model parameters section and other sections,is there a way?

04 August 2024 1,537 1 View

Which statistical test should we use?

N=6 Comparing pre and post test likert scale responses. Participants are mix of practicing & preservice teachers.

30 July 2024 7,233 4 View

What are the perfect mathematical model for a solar-powered ship implementation?

A computational study of heat flux on cylindrical surfaces using CNT hybrid nanofluids

28 July 2024 4,985 1 View

Seeking recommendations for equipment to mimic sunlight and heat up a greenhouse system?m?

I would like to conduct artificial sunlight experiments to mimic heat capture and air warming in a greenhouse system. The main objective is to heat up the temperature inside the greenhouse, not...

28 July 2024 4,006 2 View

Can you recommend provider Intermediate laboratory test for hydrocarbon, polyethylene & polypropylene?

Accredited laboratory of JV "Uz-Kor Gas Chemical" LLC (Uzbekistan) is looking for providers to conduct interlaboratory comparison tests on gas chromatographic analysis of hydrocarbons (component...

25 July 2024 8,108 0 View

Can anyone kindly suggest research topics in the area of thermodynamics, heat transfer, fluid mechanics or renewable energy?

I am an undergraduate mechanical engineering student seeking suggestions on topics related to either thermodynamics, fluid mechanics, heat transfer or renewable energy for my final year project....

24 July 2024 4,058 5 View