How to start a bioinformatic analysis of bisulfite sequencing data?

I'm just learning how to perform an analysis of methylation patterns using bisulfite sequencing data, but I have some questions: 1. What database I can use for obtain bisulfite sequencing...

10 May 2020 1,116 1 View

How to start working with epigenetic data in cancer bioinformatics?

I want to study the relationship between different epigenetic factors and the different types of cancer using existing records in epigenetic and / or oncological databases, but, as a...

28 March 2020 4,376 8 View

How can I associate an expression matrix from refine.bio datasets with its metadata?

Hi! I've been using the refine.bio website to download normalized transcriptome data; each downloaded dataset consists in a compressed directory with an expression matrix in .tsv format, its...

04 December 2019 3,635 2 View

What happens to energy prices to the final consumer by integrating renewable energy into the energy matrix of a country?

What happens to energy prices to the final consumer by integrating renewable energy into the energy matrix of a country? Do they increase, decrease, stay the same?

16 November 2019 9,316 6 View

Someone using Kraken2 for metagenomics analysis has had problems to build the plant library?

09 October 2019 9,136 4 View

Have any use the cyromazine like treatment for elasmobranchs?

In looking for a different treatment for small crustaceans that parasites sharks and rays

01 October 2019 8,772 1 View

Can someone help me with clustering DGE datasets?

Hello, I'm a biologist interested in machine learning application in genomic data; specifically, I'm trying to apply clustering techniques to differential gene expression data. I started by...

31 August 2019 8,880 9 View

Is necessary the filter in ELISA reader?

06 July 2019 6,215 6 View

How does data mining and big data differ in terms of their application?

Data mining and big data are definitely not the same in terms of definition. But how are their applications different from one another? do they even differ at all?

06 March 2019 9,412 6 View

Is there any statistical test/procedure to measure relationships among a vector (x, y, z), as explained variable, and multiple explanatory variables?

I have carried a survey to consumers. A question can be seen as a vector (x, y, z) with x+y+z=1. For example, for a consumer I have: x=0.5, y=0.3, z=0.2. Is there any statistical test/procedure to...

22 May 2017 4,833 15 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Can anyone help me with the administration of the "Mind the Gap" Scale for satisfaction with transition to adult care?

We are working on a study focused on adolescents' transition to adult care and are looking for a validated measure for satisfaction with the transition. So far the only measure that we have came...

28 February 2021 2,338 1 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View