I extract the plasmid from e. Coli, on nano drop its concentration was 60.but no band was formed in gel electrophoresis?I use 1% agrose gel with 2.5 ethidum bromid.
Do you have a MW marker on your gel does it appear ? When you say 60 in Nanodrop what do you reffering too a concentration ? Index of purity ? did you digest your plasmid with a common restriction endonuclease enzyme before to load on the gel ? Which plasmid is it ?
Jean-Pierre Dangy has asked a number of good questions, you need to be certain the gel was ok, if you saw a marker or ladder set of bands then it probably was. Secondly, lots of things will give you a reading with a nano drop, I presume you are referring to the A260. Did you do the 260/280 and 260/230 ratios to see if the absorbance you saw at 260 seems to have the appropriate profile for nucleic acid?
Lastly, if your DNA is totally degraded you will still get a A260 reading but not see any bands.
Those are the likely troubleshooting possibilities you need to consider.
On the left side in the 1st well i put the my extarcted plasmid via gaigen mini prep kit and in 2nd well gDNA of the same bacteria is present in 3rd wel i put the ladder
The gel looks very strange and the ladder is not resolved well at all. There seem to be two sets of wells, was this gel used twice?
What's the size of your plasmid? As Michael J. Benedik mentioned, did you see the bands of your ladder which will rule out the probability of an improper gel or electrophoretic conditions. Did you use nuclease free water or TE buffer? What was your 260/280 ratio? All these factors will help you to determine the actual reason of your problem. If all the above things are okay then the reason you didn't see a band is because the DNA got degraded.
My ladder bands was not very visible, and gDNA was very clear visible. Yes i use the fresh TE buffer. I will check my plasmid size.
The picture is apparently upside down as the form of the band appears inverted because gDNA should be a very high moleclular weight and sure ly not appearing as a single band usually it's rather a smear. Your gel is very badly stain or your DNA ladder is very weak
Your 1st lane from the left side has a bright band. Isn't that your plasmid ? The 2nd lane has faint bands. For how long did you run the gel ?
No 1st one has no band but the 2nd one is bright. 30mins at 100 V and 400amp
On your picture the bright band is in the Lane1 there's apparently no wells on the left side
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