Are there another copy of plasmid (circular ) of antibiotic resistance genes in DNA ??? and how to does that ???
I don't really get your question but if you explain in more details, I might be able to help.
Key features about plasmid :
1. Usually large plasmid, associated with virulence, have some mechanism to maintain a single copy of the plasmid (220 Kb of Shigella). But they are not associated with antibiotic resistant.
2. In E. coli isolates, you find two to three plasmids with different size (30-130 Kbp) that contain antibiotics resistant factors. May present in multiple copies with similar size although copy number is low (1-4 copies).
3. Additionally small plasmids (very high copy number) present that are mostly cryptic. However, a few were resistant to one/two antimicrobial agent.
To prove it, Do real time PCR (qPCR).
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0016025#s4
.Advances in synthetic chemistry now permit the chemical synthesis of single-stranded DNA (ssDNA) molecules of any sequence up to about 100 nucleotides in length. Synthetic DNA has a number of applications in recombinant DNA technology. Complementary ssDNAs can be synthesized and hybridized to each other to form a dsDNA with sticky ends. Such completely synthetic dsDNAs can be cloned into plasmid vectors just as DNA restriction fragments prepared from living organisms are.
Dear DR. this link can help you.
https://w.futura-sciences.com/sante/definitions/biologie-plasmide-4557
https://w.futura-sciences.com/sante/definitions/biologie-plasmide-4557
good luck
Hi DR.
Article Tip-Enhanced Raman Spectroscopy: Plasmid-Free vs. Plasmid-Embedded DNA
hi dr.
https://w.futura-sciences.com/sante/definitions/biologie-plasmide-4557
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