Help! I was performing a phenol purification for phenol: chloroform: IAA solution, and on what I think was my last addition of TRIS, my entire solution turned clear with no visible layering. What did I do? I saved this mixture in the refrigerator in N2 gas, do you think it is still usable?
David VanDerway
Is it possible that you removed the organic layer and then added more phenol:chloroform, therefore making it a homogeneous solution and therefore no more layers can be seen? It's likely you accidentally discarded your DNA and kept your phenol:chloroform layer.
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Laura Mason
Hi sorry, but to clarify, I was purifying the phenol from crystallized phenol; effectively reconstituting it under TRIS. Thanks for the help though !
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