I've been doing some digestion of phage DNA.
The problem I have is that some of the samples are not entering the gel matrix and stay in the well(indicated tracks 1, 4 and 5). As you can see marker runs nice and smooth, so the problem shouldn't be in the gel or the process itself. (tracks indicated as 2 and 3 are not under the question as they are from a different phage)
I´m afraid your phage DNA sample is heavily contaminated with bacterial residues, including DNA and proteins. Try a new preparation.
It seems the DNA is not purified one and may contain certain materials like bacterial cell debris, proteins or some other salts like impurities that are blocking the pore of the gel and hence the DNA is unable to move. Even you can see the DNA sample in well 2 & 3 have a restricted migration and the complete sample is unable to travel. Moreover, the gel shows formation of smear, which clearly indicates contamination in this case as the marker is well and good.
- Badges
- Science topic
- Similar topics
- Gels