Dear all,
I am working on peptides secreted from frog skin. The peptide under study is secreted from the skin of Australian frog - Uperoleia mjobergii. I would be very grateful if anyone of you could let me know what is the pH of frog skin. Thank you very much for your help.
Best regards,
Sunny
Please have a look at this useful link.
https://www.ncbi.nlm.nih.gov/pubmed/2839987
Thank you Andreas. Your info is really helpful. Do you know what low pH values you came across during the peptide secretion from frog skin? pH 7.5 - 5.5 is a good range to narrow down my working pH range for the buffers that I would like to test the peptides in for obtaining their secondary structure data.
If I know the pH during the secretion of the peptide that would be immense. Thank you once again!
Hello Andreas and Sunny,
I´m working with morphology of skin and glands of a brazilian frog -Trachycephalus mesophaeus. How are you measuring the pH on the skin surface? Are you using any specific device or any phmeter?
Thank you
Hi Adriano,
I wasn't physically measuring pH from frog's skin. I was working in vitro with the peptides that are secreted from frogs and toads. I was studying their fibrillation characteristics, so I needed to know what was the right range of pH where these single fibrils of peptides start bundling together to form aggregates. Frogs and other toads don't have a complex immune system like in the animal kingdom. So, the hypothesis is that they use their secreted peptides to help them evade any microbial attacks on their body. Some of these peptides are amyloid forming peptides and are antimicrobial in nature.
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