PFGE- What would be the wrong with my PFGE??

More Muhammad Wajid's questions See All

Please refer a primer pair for confirmation of Salmonella enterica serovar Newport through PCR??

I want to reconfirm S. Newport isolates through conventional PCR so please recommend a specific primer set for this??

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How can I represent SDS-PAGE results for machine learning analysis?

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Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

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EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

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Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

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How to manage western blot for proteomics data validation?

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Alginate and silk fibroin hydrogels toxic to cells?

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Diltuions for a Western blot?

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Why the nucleic acid gel melted after running for 30min?

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Can I get information about research laboratories that are currently having in culture, internationally/locally standardised microorganisms?

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Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

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Asst.Prof.Muthanna H. Hussain

You should visit NCBI to learn more about the time and the volts.

Muhammad Wajid

Dear Michael Hornesy,

I am using XbaI enzyme for digestion..

Savitha Nadig

Hi Muhammad,

Hope you are using low melt agarose for the run. try running PFGE ladder along with your samples. optimize on the running conditions like temperature, current and time.

best,

Savitha

Dear Savitha

Thank you for your response.. i am also using PFGE ladder with my samples .. In the above picture the mid one is ladder and its also appeared just like smear too..

Nazmul Islam

Savitha Nadig Michael Hornsey why band is not go down in PFGE test ?