I have Ovalbumin (257-264) chicken peptide from sigma dissolved in 1% Acetic acid at a concentration of 1mg/ml. I dilute it 1:100 to a final concentration of 10ug/ml in RPMI to pulse mouse bone marrow derived DC. I notice that 0.01% acetic acid may be too acidic for my cells, the media turns yellow. Anyone has experience with using 0.01% for mammalian cells? How can I neutralize the acetic acid?

More Alice Sint Thida Bo's questions See All
Similar questions and discussions