I would to know if you had the answer, because whant to knwo if I have tow similarities of 99% for one species and 98% for another species, is this enougth to stick with the first species and could we sead that the test has seperated the two species

Actually i didn't receive an answer and your two percentages are very close

I restricted my conclusion to the presence of a high similarity

Sorry if i can't give a better answer

thank you for your answer it is normal to have high similarity because they are complex species but is it significate to sead that the test is useful to seperat the two subspecies

I think you need the answer of a genetics expert. Sorry i have no certain answer

thank you so much

Sometimes percent of BLAST Identification cann`t detect the correct identification. BLAST is good for to align the similar species to measure the differences between genes . You can the test your sequences using cluster analysis or phylogeny analysis for your further confirmation. Sometime 99% similarity cann`t detect the cryptic species also. In the case of vertebrate, i always focus the morphological result first then matched with DNA barcode in blast or bold identification engine.

Thanks

Nabti, if you have sequence data from your target species/subspecies, it may be better to design a PCR reaction to discriminate between them. You just need to find conserved genes where the species are different and make primers to target them.