Hi all,
I am currently trying to label my peptides by reductive dimethylation. I was just wondering how to evaluate the labeling efficiency ? One of the way that I think of is to compare the peak areas of both labeled and unlabeled peptides after labeling. However, I am not sure if that is correct as the labeling can change the peptide's ionization because of better proton affinity, hence; it is unfair to compare the labeled and unlabeled peptides. Does any one has any other ideas?
Thanks a lot.
Eef Dirksen
Hi,
You could compare the peak area of any residual unlabeled peptide to that of a separate injection of an unlabeled peptide sample (i.e. starting material).
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