6 Questions 9 Answers 0 Followers
Questions related from Trung Tran
Hi all, I am currently trying to label my peptides by reductive dimethylation. I was just wondering how to evaluate the labeling efficiency ? One of the way that I think of is to compare the peak...
07 July 2018 8,886 1 View
Hello you all, I am trying to isolate nuclear extract to study the transcription factors. I am mainly working with cell lines, I have found two kits for this....
06 June 2018 5,043 7 View
Hello, I am planning to enrich O-GlucNAc proteins in Jurkat T-cells by metabolic labeling before performing drug-treatment with Cisplatin. I have some practical questions regarding this: 1. Is it...
10 October 2017 7,232 0 View
I have tried to identify and enrich phospho-peptides by using TiO2 beads (control vs cisplatin treated Jurkat T-cells). Then I searched all the raw files using Proteome Discorverer 1.4 against...
09 September 2015 9,853 4 View
Dear research gate members, I am trying to detect all the members of the BCL-2 protein family in T-cell extract. However, normal procedure in proteomics experiments did not work efficiently....
12 December 2014 9,062 4 View
Is there any one know the average amount of proteins obtained in a single Jurkat T-cell? Thanks
10 October 2014 8,119 5 View