Hello-
I have left 100x pepsin solution at 4 degrees for a couple of months. Now I realize it should have been in the -20 degree. Has the pepsin gone bad? also, is it ok to store pepsin at that concentration? It doesn't necessarily stay in solution, so I have to mix it pretty well every time I take some.
Thank you,
Kate
most of the enzymes are inherently unstable in aqueous medium if y keep for a longer period of time. Certainly many factors involved in solution stability are like pH , ionic strength, any additive/preservative etc etc. If you ar not sure of the stability of ur enzyme, it is best to check the activity of enzyme and compare with the previous data or with specifications. I would suggest you to prepare a fresh solution instead. Proteins are best stored in lyophilized/powedered form. Dry forms you can keep even at 4 degree for a number of months.
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