How could the concentration from RNA extraction is really low?

Hello everyone, currently, I'm trying to do RNA extraction from seeds of Arabidopsis. I'm using the protocol below: RNA isolation from siliques, dry seeds, and other tissues of... I use about...

17 February 2021 7,583 4 View

How do I measure Arabidopsis germination rate?

Hi there, I'm looking for a way to measure the Arabidopsis germination rate, like what media I should use to plant the seeds and what indicator I should see for germination. I'm planning to...

02 February 2021 4,785 3 View

SDS-Gel stacking gel polymerization issues?

Can anyone provide any insight as to why my Tris/Gly SDS-Gels are polymerizing slowly? This especially applies to my stacking gel (image attached) both 4% and 6%. The stacking gel wells polymerize...

24 January 2021 3,791 4 View

How to use back-trajectory analysis (HYSPLIT) to assess air parcels at breathing/monitor height?

Hi there, I'm currently monitoring air pollution in Grenada, West Indies and as part of my project I have identified four scenarios in which I would like to use a back-trajectory analysis...

27 December 2020 730 4 View

Origin of the term "transposition" used in photovoltaic performance modeling?

In PV modeling, the process of elevating GHI data to a tilted surface is often referred to as "transposition". However, strictly speaking, "transposition" in mathematics refers to rearranging a...

22 September 2020 767 3 View

Help figuring out why only lifted cells express eGFP after transduction?

I'm attempting to transduce HEK293T cells with a lentivirus expressing eGFP. I have been observing a low transduction efficiency and after a 24 h transduction the only cells expressing eGFP are...

03 September 2020 9,491 5 View

How to stimulate PBMC-derived monocytes with TLR7 and TLR8 agonists?

I'm trying to stimulate human monocytes from fresh PBMC isolations with 2ug/mL imiquimod and 2ug/mL ssRNA40. I do this in 10% FCS complete RPMI for 18-20 hours before staining and analysing CD14+...

10 August 2020 7,095 1 View

What are the independent, intensive parameters for multicomponent mixtures (and how do I determine them)?

Hi all, For different problems in gasdynamics/thermofluids, different thermodynamic inputs are most convenient for expressing and solving the problem. For example, if I have a stagnation process,...

23 June 2020 1,085 2 View

What literature should I read to start understanding flow batteries?

I need recomendations on the most core and fundamental materials/literature to start understanting flow batteries from scratch so I can migrate to membraneless flow batteries. Hence, I will also...

09 June 2020 6,270 3 View

Any recommendations for a high-quality, contract basis, academic illustrator for a paper in Nature Climate Change?

We looking for a high-quality, contract basis, academic illustrator for a paper in Nature Climate Change, any recommendations? Proven track record important.

26 April 2020 7,427 8 View

Negative Inhibition in Confirmatory ADA assay?

I am developing an Anti-Drug Antibody assay and keep getting a negative percent inhibition (negative control sample results are higher in confirmatory than in screening format) when running...

19 August 2020 6,658 1 View

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18 May 2020 6,271 2 View

What are some examples of practice based methods and techniques that can be used to study social dance styles?

I am currently developing a project where the primary mode of inquiry is rooted in practice based methods and techniques. The project is focused on a social dance form. I have read several...

23 March 2020 793 5 View

How does being a teacher influence creative processes in creating choreography?

I would be interested in resources that pertain to how identity or the different roles we possess in dance making (dancer, teacher, spectator, mentor, etc.) influence creative processes in...

06 December 2019 8,064 2 View

Can two established different species have more than 96% similar DNA sequences?

Some times two plant species which are morphologically different have similar DNA sequences. What should be the final conclusion?

08 September 2019 1,655 7 View

Ecological Validity: How can experiments that take place in a social setting measure validity and reliability?

Thinking in terms of a social setting such as a dance, a concert, a meal, if an experiment were to be designed in such a way, how can the method be validated? Similarly, what role would...

26 August 2019 3,415 3 View

Where can I find articles about emotional education programs for teachers that involve the body in an educational context?

Hello everyone, I am interested in the role of the body (or corporal dimention) in the educational context, specifically the interactions between teacher and students during the teaching-learning...

07 August 2019 2,805 5 View

Ecological Validity: How can experiments that take place in a social setting measure validity and reliability?

Thinking in terms of a social setting such as a dance, a concert, a meal, if an experiment were to be designed in such a way, how can the method be validated? Similarly, what role would...

07 August 2019 2,662 3 View

Will more social prescribing save money?

Will more social prescribing save money? Social prescribing: coffee mornings, singing groups, and dance lessons on the NHS BMJ 2018; 363 doi: https://doi.org/10.1136/bmj.k4857 (Published 19...

05 June 2019 9,732 1 View

Where can I find or contact to get infos about deaf federal workers database?

I am hoping to get most updated infos about data on deaf federal workers/employees that got hired, got rejected, sued/cases against federal agencies for violated ADA rights and more. Thank you

05 June 2019 6,461 4 View