I am doing a very simple fragment analysis for quantitation of two oligo fragments using PeakScanner. However, I am frequently running into some problems. The software does not correctly recognize some of the dye standard peaks, and therefore does not output the sizes for the fragments of interest. I am using .fsa files generated by an ABI instrument.
Will someone with expertise in this software be willing to help me out by looking into a small sample file?
Thanks
It may be that you are not adding enough size standard and on some peaks the peak height is below the Peak amplitude threshold so the peak is shown but not analysed by the software. Editing the Peak amplitude threshold to a lower value for either all or just one sample might work. Page 46 of the attached document may be of interest. Sorry there seem to be legal issues with sending this so the manual is found here
Sizing Analysis Module Peak Scanner Software User Guide; Pub. No. MAN0017576, Rev. A.0 (thermofisher.com)
- Badges
- Science topic
- Similar topics
- Computer Science and Engineering
- Programming Languages
- Python