PDX1 antibody in mouse pancretes is not working, ideas suggestions?

09 August 2017 0 9K Report

pancreatic Duodenal Homeobox1 protein is a intranuclear protein and I need to stain for it in mouse pancreates by immunofluorence. I have used Abcam and CST antibodies follow attached link protocol and their not working, For me, I use to go 1：200 dilution for primary Ab and 1 : 500 for secondary Ab（abcam)and the thickness of tissue section is 6-10um.Both paraffin section and frozen section was used in experiment ,but pdx1 can not detect by immunofluorence....i have changed the permeabilization states as well as the concentrations for the antibody, has anyone used PDX1 in mice and if so what concentration and the protocol? Help please!!

https://www.abcam.com/ps/pdf/protocols/double%20immunofluorescence%20-simultaneous%20protocol.pdf

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