Hi all,

I am doing lipofectamine transfection. Still no working. I have a doubt regarding the purity of DNA. My pDNA was obtained from Midiprep, but in the last step I diluted too much. To fix that I did DNA precipitation.

The protocol for DNA  precipitation is simple:

I took 2ml of DNA (from my midiprep with low concentration because was too much diluted) into a microtube

However, I am concern if doing this could affect my future experiments, like transfection with lipofectamne, because salt contamination or something related.

My pDNA had the following results from the spectrophotometer:

A 260 =28.522

A280=15.2

A260/280=1.67

Please, let me know your thoughts, I wait for your instructions.

Best,