PCR primer designing

More Syed AHMED Rizvi's questions See All

How to we see transcription activity of the bhlh79 family is at the N-terminal or C-terminal?

I'm a PhD Student. From Northwest Agriculture and Forestry University China. My Problem is this I start my work on bhlh79 transcription factor gene. I do my Y2H 5 time but the colonies appear on...

30 July 2024 1,412 8 View

HEC 1A & HEC1B Cell Lines?

Hi, Kindly guide me that how many cells of HEC1A & HEC1B Cell lines should I seed for Wound healing assay and which plate type is recommended 6, 12 & 24?. Articles suggested mainly 24...

20 July 2024 4,143 2 View

How to determine the quantity/amount of secondary phase based only on peak intensity?

I have 3 different specimens. Each specimen has a main peak of the targeted phase, however, all of them also have a secondary peak of associated with a secondary phase. Now just based on the...

26 June 2024 5,049 5 View

How can machine learning be employed in the field of natural sciences?

Hello! I am pursuing Ph.D. in Environmental Sciences from Sambalpur University, India. My thesis topic broadly focuses on physico-chemical analysis of water and soil samples. I eagerly want to...

18 June 2024 1,897 4 View

GRADE approach in physiotherapy paper?

Can anyone guide me where I can find and understand the GRADE approach method for my ongoing systematic review? I referred to Cochrane and it's way too complicated to understand. Thank you in...

15 June 2024 6,393 2 View

Can someone help me with the alpha amylase inhibition assay?

I am determining the alpha amylase inhibition activity of my methanolic extracts by DNSA method. The problem is, my sample absorbance is higher than the negative control which made the final...

27 May 2024 9,348 0 View

How can I find permission and access to parenting style questionnaire?

I need to use and adapt the parenting style questionnaire in my ongoing research project, can somebody please help me regarding gaining permission and access to them as I am unable to find the...

25 May 2024 6,304 1 View

Cell Culture Debris? or Contamination?

I'm doing OSCC cell culture and routine media (supplemented MEM) changes every 72 hours. The media looks healthy in terms of color (reddish-orange), and there was no microbial contamination...

12 May 2024 9,279 2 View

How to improve RNA Integrity in bacteria using Purelink RNA mini kit?

I am using Purelink RNA mini kit (Fischer Scientific) to extract total RNA from bacteria. i need to get better RNA integrity number like more than 7, but for only one sample I got 7.4, for other...

01 May 2024 4,355 3 View

How to simulate Wall shear stress in COMSOL?

Water flows in a 2D pipe, I am able to prepare a simple simulation resulting in the velocity and pressure across the pipe. But I am interested to simulate the Wall shear stress generated by the...

22 April 2024 8,537 5 View

• What the possible Persistent Organic Pollutants and Heavy metals present in fluorspar, sediments, and water bodies around its mining area?

Approximate concentrations are require in compared with the WHO permissible limts

11 August 2024 2,723 1 View

Handling Missing Data and Building a Predictive Model with Incomplete Information ?

I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...

10 August 2024 4,005 2 View

Do interactions between biosphere, carbon cycle, & water cycle impact global warming & interaction between atmosphere & hydrosphere?

How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?

09 August 2024 3,291 2 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Is it true that $\det(V(A))$ may be only $\pm 1$, depending on $n$, for the last symmetric tridiagonal matrix $A$?

One can try to generalize the Vandermonde determinant in the following direction: Let $A$ be any symmetric $n$-order square matrix. Consider its powers' diagonal elements $(A^k)_{ii}$ and...

08 August 2024 6,690 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks

06 August 2024 949 0 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Rohit Kumar

Water sample collected and bacterial DNA has been isolated. And thus primers are required to confirm the presence of antibiotic resistance genes.

Bandla Ramesh

yes required.The primers for antibiotic resistance genes vary depending on the specific gene that is being targeted. It is best to consult the scientific literature or contact a laboratory that specializes in gene sequencing to find the appropriate primers to use for the specific gene in question.