PcDNA3 linearization

More Philippe Bouaziz's questions See All

Streptavidin Coated Beads

Dear All, Any one having protocol to covalently attach Streptavidin Coated beads to Lambda-DNA. For your information: Lambda DNA has a sticky end. Thanks a ton!! phil

02 March 2011 4,556 2 View

embryoid body seed on PLGA scaffold analysis

Hey all, can anyone give suggestion of how to degrade PLGA scaffold in order to do qPCR? and also how to visualize the cells in the scaffold by microscopy because the scaffold is non transparent?

04 May 2010 2,218 2 View

How to destroy BET?

Do someone know how I can clean bet without risk?

11 December 2009 1,907 0 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View

How to analyse a (perceptual) study using differing/comparable sets of stimuli?

I plan on running an online perceptual experiment that has, let's say, 100 unique video stimuli altogether. Since these are too many to be rated by every participants, I would like to present the...

15 February 2021 7,444 2 View

How to analyse a split-plot design in stata ?

I have run an split-plot design experiment evaluating the efficacy of a treatment (with 3 whole plots and 4 sub-plots), and I am planning to analyse the collected data in Stata. However, I do...

14 February 2021 7,800 7 View

Can I use a likert survey for criterion sampling in a qualitative study or would that make it mixed methods?

I am doing a phenomenographic study on the reading experiences of avid v. Neutral v. Non-Readers. If I use a likert survey to criterion sample for participants, does that make it a mixed method...

09 February 2021 8,594 3 View

How to create the context-mechanism-outcome configuration (CMOc) in the realist methodology?

In the realist methodology (especially by Raymond Pawson et al), the CMOc is a common structure to explain what works, for whom, in what circumstances, and why? I'm always asked about the...

08 February 2021 2,792 1 View

Recommendations for sample size in visual qualitative research (using children's drawings)?

Hi everyone! Asking for a little help, if I may. I am working on a research project, where I aim to analyse children's drawings about Covid-19. I am fairly new to using visual data in qualitative...

07 February 2021 9,530 3 View

How do I create a categorical variable, level of exposure, and a dummy dichotomous, yes-no, variable for the Harvard Trauma Questionnaire Part 1?

I am using the Harvard Trauma Questionnaire (HTQ) Trauma Events scale (original version) that has 17 items (17 different traumatic events) with 4 response options, indicating 4 levels of exposure:...

04 February 2021 1,509 3 View

How to report path analysis / SEM with Yuan-Bentler correction from R output?

Dear all, I am currently writing my master thesis where I am analysizing my data using path analysis and SEM in R (lavaan package). Now that I like to write up my results, I am struggling with...

04 February 2021 8,993 2 View

Philippe Bouaziz

yes but if I don't cut 100% of my plasmide and there is circular DNA,supertours DNA and also linearize DNA

how can I know what will be my linearize DNA?

good explanation ,now I'm trying to digest my pcDNA3 (8000kb) by bglII unfortunatly after 1h digestion I can't desactivate the enzyme by heat do you know how can I desactivate it and what can happen if I just don't desactivate the enzyme ?

I have another question I'm loading 6µ(5µl DNA +1µl loading dye)l of my DNA in order to have a better profile of my gel can I load just 6µl of my leader to what your generally doing?

thankyou for your help!!

phil