PAR-CLIP - FAQS.TIPS

08 August 2014 0 2K Report

I have a few questions regarding PAR-CLIP

1. What is the typical yield of RNA after its recovery from the polyacrylamide gel ? How I can check that the gel-recovered RNA is enough for cDNA library preparation?

2. This method is routinely done with P32 radioactive labeling of RNA to visualize it on the polyacrylamide gel. Does anyone has an experience or a protocol for doing it in a non-radioactive manner ? Can fluorofor labeling help?

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