P24 tests with Blood and Urine samples?

Saikat Chakraborty @Saikat-Chakraborty-3

02 September 2022 1 7K Report

Do we need immune complex disruption steps in Blood and Urine samples for p24 ELISA tests?

You would probably be better off developing a quantitative PCR rather than trying for a P24 antigen capture. If the sample comes from blood then anti-p24 antibodies are always a possibility for interfering with the assay. In Urine you will have to cope with the pH.

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