Is it suitable to stabilise human blood cells by RNA-Later to perform DNA expression studies?

Human blood cells stabilised by RNA-Later to perform DNA expression studies. It is a good way to store white blood cells to perform subsequently the isolation of RNA? Or is it preferable to store...

23 June 2015 3,340 3 View

Can someone give me a suggestion on the cloning procedures?

11 March 2015 1,728 4 View

Does anyone have experience with, or literature on, bone plow or rake phenomenon using locking plate fixation ?

When bone is cut by locking screws. In my experience in metaphyseal bone.

08 February 2015 5,366 2 View

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I have a list of genes annotated with InterProscan. I would like to summarize the results plotting the GO category and look at which major function or biological processes those genes are involved...

12 August 2014 455 2 View

Who knows sites hosting free samples of images with known clusters inside to test clustering machine learning models?

I have several clustering models to be tested and validated on images for which clusters inside are well known.

23 March 2014 7,277 2 View

Could somebody please give me the nucleotide sequence of the TEV protease recognition site?

I would like to use this sequence for TAP of protein complexes.

17 February 2014 7,498 7 View

Which activities should be the most important for academic people?

Normally an academic dedicates do some hours per week to research, some to teach and some to administration of the Department or Faculty. Some hours are also dedicated to external work, as...

01 November 2013 4,783 3 View

Can anyone advise on antibodies against "histone locus bodies" that work in imunofluorescence?

I am trying to identify histone locus bodies in a prostate cancer cell line (PC-3)

09 July 2013 280 3 View

Hek-293 stable cell line problems?

I am having a problem with the selection of my HEK-293 cells. I started the selection with G418 two months ago and I still have in my flask untransfected cells that don't die. What can I do? Try...

20 June 2013 1,849 19 View

What are the best markers for the identification of Neutrophils, eosinophils and circulating dendritic cells in whole blood by flow cytometry?

I need to study these cells in whole blood samples. I normally use the protocol "Lyse-no-wash". It could be used to study these cells, too?

05 April 2013 5,771 10 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View

Are these moths Spodoptera frugiperda?

The caterpillars of the moths were collected as fruitborers in pepper at a farmer. I reared them till the adults hatched and compared them with pictures and descriptions on the internet. Are these...

24 February 2021 7,785 5 View

Steps to calculate activation energy by coats & redfern method?

I'm a chemistry student and I'm researching the kinetic degradation of mixed plastic waste. I'm having trouble calculating the activation energy by the coats & Redfern method. The data that...

24 February 2021 974 1 View

Any Differences in COVID-19 vaccines?

Are there any differences (outside of the clinical trials that led to their EUA) observed in the rate of infection among those who have received the Pfizer vs Moderna vaccine since the mass...

22 February 2021 9,727 6 View