The most common model is Subserosal injection of acetic acid (20%, 0.02 ml) into the middle colon of rats. The size of the induced ulcer could directly be measured using a caliper gauge, and the result was expressed as the ulcer area (mm2).
Oxazolone-induced colitis in BALB/C mice: a new method to evaluate the efficacy of therapeutic agents for ulcerative colitis.
Hi
you can use the DNBS for the induction of Colitis by the intracolonic administration of DNBS similarly to (Cannarile et al., 2009). Briefly, mice were anesthetized with halothane, and DNBS (3 mg/mouse dissolved in 0.1 ml ethanol/water 50% v/v) was inserted into the colon using a polyethylene catheter via the rectum, and maintained in down right position until recovery from anesthesia.
Using DNBS or TNBS causes symptoms similar to the crohn's Disease.
Best regards
Hello Amina,
2,4,6-trinitrobenzene sulfonic acid (TNBS) and DSS are probably the most commonly used agents to induce colitis.
There's one example of sodium hydroxide being used (Kocak et al, Dig Dis Sci. 2011 Oct;56(10):2833-7. doi: 10.1007/s10620-011-1697-8), although this is claimed to be more similar to Crohn's disease than ulcerative colitis. Also because it's the only example I'm aware of, it may not be appropriate.
Note that TNBS and DSS are both referred to as models of either UC or Crohn's disease (depending on the reference), so you should be clear about which specific symptoms you are interested in and be sure that the model you choose is likely to have those symptoms.
Finally, these agents do not actually induce UC. UC is a human disorder, and while there are some good models available they are just that - models. They will therefore not cover all aspects of UC. You should be aware of the limitations of any model you do choose, and I would suggest you always refer to it as a "model of human UC".
I hope that's helpful, and good luck!
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