Hello, everyone,
Has anyone been successful in isolating good quality exosomes using an OptiPrep density gradient? I have been trying different modifications in the OptiPrep protocol so far to isolate exosomes from serum, but with not much success at all. Could someone let me know if there is a working protocol to effectively isolate exosomes from serum? I have tried the classic UC, and have been able to validate them using Westerns and they have worked fine, although the yield has been very low. Please recommend any protocol or a combination of protocols that could be used to isolate good quality exosomes not including the precipitation process?