Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

Pls inform me if the problem here is in the used red safe. im using the red safe of vivantis co, Malysia. using 5 microliter per 100 ml of agarose?

Why the ladder is always with so closle or attached bands after the 500 bp band whatever i use a type of ladder ( 5types) and also the PCR product is bended. is this has a relation with the used...

05 January 2021 5,211 5 View

Is it important/advisable to write a summary for each theme (and subthemes) in Thematic Analysis?

I am conducting semi-structured interviews with graduate students to explore their academic writing challenges. I am using thematic analysis to analyze the Qual findings. Should I include a...

31 December 2020 3,593 22 View

Using Matlab and the analytical solution for Room acoustic modeshapes, is it possible to plot modes in 3D?

mode shapes similar to attached image.

27 November 2020 4,262 7 View

What tool can I use for creating a Quantative Trait Loci map from SSR data?

I have SSR data for some individual of three hybride How I construct QTL mapping of yield according to these data ?

02 October 2020 502 5 View

Labs for purification, identification & isolation of unknown proteins form native sample of honey and to test versus GS-9L cells in vitro?

I asked for protein purification, identification and isolation of unknown proteins and test versus GS-9L other than that we already got in our patent WO/2014/040605 - form native sample of honey?!

23 September 2020 1,839 3 View

Hi everyone, do you know any GPS logger that we can use to conduct travel time surveys and which is easy to extract the data from ?

We are currently planning to conduct Freight Master Plan in the state of Qatar, one of the requirements is to conduct travel time surveys, therefore we are currently searching for GPS devices that...

22 July 2020 1,889 1 View

How can I quantitatively analyze a sample with carbohydrate content?

Can anyone -who may have hands-on experience in analytical chemistry or photochemistry- help me how can I quantitatively analyze a sample with carbohydrate content?

09 July 2020 3,663 2 View

Any Predictive Markers for Severe COVID-19 in children?

Predictive Markers for Severe COVID-19 Identified

15 June 2020 1,375 5 View

How to critize (Evidence based medicine) this paper?

24 May 2020 5,489 3 View

What will happen when we assume an upper bound of OFV lower than the global minimum OFV ?

if we assume 20 is the upper bound of an objective function (OF) based on permutation a square matrix (nxn) , The minimum value of the OF=22, then we run a random operator to compute the OF by...

14 February 2021 6,056 5 View

Best loss function for special classification problem in neural networks ?

Hello, I've build a Conv1D model to classify items into 6 categories (from 0 to 5). (simplified example) I use therefore a CrossEntropy loss function that penalizes the errors with high...

19 January 2021 3,199 1 View

How can to calculate the Computational complexity/cost (espatially, number of operations and space/memory cost) for an algorithm?

As we know, computational complexity of an algorithm is the amount of resources (time and memory) required to run it. If I have algorithm that represents mathematical equations , how can estimate...

25 November 2020 6,950 7 View

Why isn't my quantum espresso computation converging?

I am a beginning with computation, and this is my first time working with an organic molecule.I have attached my input and output files. I was able to get the organic portion of the molecule...

19 November 2020 10,004 3 View

How to compute the horizontal and vertical velocities (involve in the subduction rate computation) from Argo data of Temperature and Salinity?

The goal is to compute the total/annual subduction rate of mode water. For this computation, we need horizontal currents (u,v) and vertical currents (w) at the base of the mixed layer when the...

04 November 2020 5,239 3 View

How to compute the effects in half fractions?

Greetings, I'm doing a screening design and want to allow for lack of fit. I need replications so I want to add four additional points to my dataset. Rather than use center runs I use corner...

11 October 2020 9,672 1 View

Please suggest any user friendly pipelines for the computation of Polygenic Risk Scores (PRS) on Microarray datasets?

I want to analyze the PRS scores for my microarray datasets, but want to use a platform which is a bit user friendly. Please suggest for any pipelines available.

06 October 2020 5,691 4 View

What is the best language for symbolic computations?

In finite element method, especially when developing new elements, we often need integration techniques. Generally, numerical integration is used, however, in order to enhance the accuracy of our...

12 August 2020 997 19 View

How the parameters affect the quality of solutions and the computational effort required by the Swarm Intelligence algorithms?

What is the effect of increasing or decreasing population size and the number of iterations on the quality of solutions and the computational effort required by the Swarm Intelligence algorithms?

24 June 2020 8,540 13 View

Is it always better to use friction values in inundation modeling, even when we have high-resolution DEM, where rough surface changes are detailed?

I am aware of computational costs while working with high resolution data, so this question is more of hypotetical nature and is related to the relevance of friction parameter in inundation...

23 June 2020 2,172 2 View