I got the result from those region. If possible?
I have used two regions of insert one is 1.3kb and another one is 2.7kb. It has not shown positive result for past two months.
08 September 2012 4,107 2 View
I am using fermentas T4 DNA ligase enzyme for ligate binary vector with 2.7kb insert. But it does not show any positive colonies. How can I check that enzymes is working?.
07 August 2012 1,140 2 View
I have ligated 1.3kb fragments to binary vector but it is not showing any single colony after transformation. What could be the problem?
07 August 2012 869 7 View
I have done PCR many of the times with the same DNA, sometimes I got good results, sometimes they did not show any specification. What is the problem for this condition or do I need to change any...
06 July 2012 4,241 12 View
I want to know about primer designing for RNA interference. Which region should be selected for efficient target degradation? I am working with ssDNA geminviruses.
06 July 2012 7,984 0 View
I want to know about the release of an insert from a vector. I am using two restriction enzymes for release of insert. It binds with a vector containing restriction sites. How can I release that one?
06 July 2012 4,265 3 View
I want to know about binary vector cloning, because many binary vectors are using to our research purpose. Whether it is possible to know binary vector construction?
06 July 2012 588 0 View
I'm very interested in biotechnology and medical research and I'd like to meet a professional here and talk about it together
28 July 2024 3,798 4 View
List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?
27 July 2024 4,800 4 View
I am pleased to announce the CUSABIO Biotechnology Scholarships for Students 2024! This scholarship aims to support outstanding students pursuing studies in the field of biotechnology. Here are...
22 July 2024 5,790 1 View
I was wondering if it is possible to form a permanent open "ssDNA bubble" similar to a transcription bubble (>13 nucleotides) within E. coli. These criteria are important: 1. Open ssDNA...
07 July 2024 8,275 5 View
Give in detail with available literature and website links for submissions
27 June 2024 9,753 1 View
Hey everyone! I'm having difficulty marking the C4 complement factor in Western Blotting. Has anyone had contact with this antibody or made it in-house? I have already used Anti-C4β (D-12)...
17 June 2024 6,124 1 View
Generally for bacterial DNA isolation we use a fresh culture of 2-5 days old. But if we use an older culture say 10-15 days old will it have any impact on the DNA content and the DNA isolation...
13 May 2024 3,061 11 View
I was curious as to why modern soybean half-seed protocols use 1/10x strength B5 salts during the co-cultivation step rather than full strength B5 salts? Also, are the B5 vitamins added at full...
07 May 2024 3,924 0 View
I am following the standard method which is mentioned in Tomotake Kanki et al., 2009 research paper Monitoring mitophagy in yeast: The Om45-GFP processing assay . I am growing the cells in SD-HIS...
04 May 2024 6,463 0 View
How are these exploited in biotechnological applications?
07 April 2024 566 5 View
