npt II gene is possible to detect siRNA in plants.

I got the result from those region. If possible?

07 August 2013 1,340 0 View

How can I overcome binary vector ligation troubleshooting?

I have used two regions of insert one is 1.3kb and another one is 2.7kb. It has not shown positive result for past two months.

08 September 2012 4,007 2 View

How to check whether T4 ligase enzyme is working?

I am using fermentas T4 DNA ligase enzyme for ligate binary vector with 2.7kb insert. But it does not show any positive colonies. How can I check that enzymes is working?.

07 August 2012 1,037 2 View

Problem with binary vector cloning. Is there any method available to check for our cloning product?

I have ligated 1.3kb fragments to binary vector but it is not showing any single colony after transformation. What could be the problem?

07 August 2012 775 7 View

Could anybody help me with PCR troubleshooting?

I have done PCR many of the times with the same DNA, sometimes I got good results, sometimes they did not show any specification. What is the problem for this condition or do I need to change any...

06 July 2012 4,059 12 View

How to select the sequence for designing siRNA?

I want to know about primer designing for RNA interference. Which region should be selected for efficient target degradation? I am working with ssDNA geminviruses.

06 July 2012 7,903 0 View

Restriction enzymes digestion for release of insert from vector?

I want to know about the release of an insert from a vector. I am using two restriction enzymes for release of insert. It binds with a vector containing restriction sites. How can I release that one?

06 July 2012 4,157 3 View

No title

02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

What HPLC Marker(s) should be used to detect anthocyanins in a sample?

What HPLC Marker(s) or standard is used to detect and quantify anthocyanins such as Delphinidin, Cyanidin in a given sample of plant extract?

23 February 2021 1,832 4 View