Deivamani Mariyappan

8 Questions 14 Answers 0 Followers

Questions related from Deivamani Mariyappan

npt II gene is possible to detect siRNA in plants.

I got the result from those region. If possible?

08 August 2013 1,407 0 View

How can I overcome binary vector ligation troubleshooting?

I have used two regions of insert one is 1.3kb and another one is 2.7kb. It has not shown positive result for past two months.

09 September 2012 4,075 2 View

Problem with binary vector cloning. Is there any method available to check for our cloning product?

I have ligated 1.3kb fragments to binary vector but it is not showing any single colony after transformation. What could be the problem?

08 August 2012 839 7 View

How to check whether T4 ligase enzyme is working?

I am using fermentas T4 DNA ligase enzyme for ligate binary vector with 2.7kb insert. But it does not show any positive colonies. How can I check that enzymes is working?.

08 August 2012 1,111 2 View

Restriction enzymes digestion for release of insert from vector?

I want to know about the release of an insert from a vector. I am using two restriction enzymes for release of insert. It binds with a vector containing restriction sites. How can I release that one?

07 July 2012 4,231 3 View

How to select the sequence for designing siRNA?

I want to know about primer designing for RNA interference. Which region should be selected for efficient target degradation? I am working with ssDNA geminviruses.

07 July 2012 7,960 0 View

Binary vector cloning

I want to know about binary vector cloning, because many binary vectors are using to our research purpose. Whether it is possible to know binary vector construction?

07 July 2012 565 0 View

Could anybody help me with PCR troubleshooting?

I have done PCR many of the times with the same DNA, sometimes I got good results, sometimes they did not show any specification. What is the problem for this condition or do I need to change any...

07 July 2012 4,183 12 View