Hello all,
Can someone recommend me a range of doses of Doxorubicin to induce a nephropathy model in BALB/c mice? I searched the literature and it seems that Doxo dose should be set very sensitive and even the batch of Doxo can affect the result :( I used Doxorubicin Hydrochloride from Santa Cruz Biotechnology. Cumulative 15, 16 and 20 mg/kg (ip) doses kill the animals. However, 9.6 mg/kg (iv) and 10 mg/kg (ip) doses didn't yield a significant difference (in 23 days). I'll make my last trial, I don't want to lose more animals. Should I change the brand of Doxo? Is there a limit (such as 12 mg/kg etc) where the lethality (cardiotoxicity) begins? Should I wait more than 4 weeks to get a cumulative injury on kidneys?
Anny other recommendations?
Best
Hi,
Since IP injection of Doxo simple, but locally very toxic for intestine and thus resulting in animal loss. Our collaborators performed pilot study to optimize Doxo dose in C57BL mice for inducing cardiotoxiticity. The best results obtained from tail vein injection. As for kidney weight (mg) to tibia length (mm) ratio, it was reduced in comparison to control by 10-20 and 20-25 % for Doxo dose of 4 mg/kg and 20 mg/kg respectively 9 days after injection in both sexes.
The most important things to control water availability since animal very weak after injection and sometimes not able to use central water supply system or bottles. So put transportation gel or just food pellets soaked with water available for animals.
Hope it will help to improve your experience with this model.
Daniil
Thanks Daniil R. Petrenyov
I also tried 2X10 mg/kg IP on C57BL6, again mortality was very high and I couldn't get a significant damage in kidney histology of the survivors. Did your collaborators see any other difference in the kidney (histological, biochemical or any other parameter)? I can think about changing the way of injection.
Consider increasing the exposure duration for either of the non-lethal doses since manifestation of toxic influences is also a function of exposure duration.
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