What should be the platform of your choice, if you are doing somatic mutation study, using ION platform
I really need some good suggessions... Thanks
Is there any option to check somatic variant, by the use any probe based technologies?
I think you can use allele specific PCR, or if the mutant allele frequency is not too low even Sanger sequencing can give you a quick validation.
According to market study performed by Ghent University spin-off company pxlence, 56% of scientists (n=200) validate their NGS findings with Sanger sequencing. pxlence has PCR assays for the entire human exome.
https://www.pxlence.com
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