Dear researcher,

I am currently studying the kinetic of of soil urease with and without the urease inhibitor. Trying to figure out the Km and Vmax values. However, when plotting my data for lineweaver bulk , I got negative intercept. have repeated the assay few times, but still same results.

Probably there might be an error on how I plot the data and there is a better way to do it. Hope u can share it

The way I plotted was using excel and extend the trend line.

Also attached the chart that I plotted and raw data.

Thank you

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