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More M. P. Hendawitharana's questions See All

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

How can I homogenize this data to make a meta-analysis?

Hi, I am doing a review and hopefully meta-analysis for my Master thesis but I have the following problems: - Some studies only give the difference between end trial and baseline data and other...

22 February 2021 1,115 4 View

Research Gate Does it help researches to publicize their scientific work ?

In my opinion, the RG site is useful in solving scientific problems and shows the researcher's ability. Besides, the separation of the H-index from the self-citations and the overall index is an...

22 February 2021 3,894 6 View

Monte Carlo simulation?

Hi, i am conducting a Monte Carlo simulation for my new distribution but the MSE values are increasing while the sample size increase, does any experience this issue before and what should i do...

22 February 2021 3,680 4 View

Which is the best software or programming language (user friendly) for analysing data?

I have a basic knowledge on Python, R and MATLAB in analysing data. Among those, which is the best and user friendly? Which one should I learn deeply to analyse data?N:B: I want to learn data...

20 February 2021 3,950 15 View

Is There are any idea to achieve H NMR signals for swollen compound ??

the samples are not completely soluble - just swelled the solvents that's mean the sample may be partially soluble any suggestion, please

16 February 2021 8,801 3 View

How many samples can be pooled together for NGS?

We collected faecal samples from various species that we want to analyse their microbial communities' DNA via Next-Generation Sequencing. The problem is that due to budgetary constraints, we will...

16 February 2021 5,466 4 View

Flexibility of RNAseq?

When you use RNAseq I understand that it must follow certain conditions when you use it. One of them is the insertion of a polyA tail. The insertion of the polyA must include then...

05 February 2021 3,770 4 View

How to identify when a gaussian program is about terminate normally?

Dear all, I am trying to optimize a bimetallic complex using # opt=tight b3lyp/lanl2dz scf=qc geom=connectivity. The program is running for around 5 days but the size of the .chk remains same...

05 February 2021 8,721 3 View

How to enter a variable with multiple selections as response in SPSS software?

Hello I wanted to ask how can I enter a variable in SPSS with the option of multiple responses for a given variable? For example: If question is "What is your role?" Answer Choices: (A) Positive...

08 January 2021 5,741 4 View

Data to estimate Potential Fishing Zone?

I need data to estimate Potential Fishing Zone .The data related to any of the attributes like dissolved oxygen, sea surface temperature, chlorophyll and salinity in numerical form with respect to...

25 December 2020 8,515 4 View

Does Linear Discriminant Analysis (LDA) assess linear relationship between independent variables and variables used for grouping?

I grouped sites in six different clusters on the basis of fish species abundance using a self-organizing map (SOM). Now, I want to determine to what extent water chemistry variables can explain...

19 September 2020 8,903 3 View

Do you know of publications reporting fisheries post-release survival rates of marine turtles?

We are compiling publications that estimate marine turtle survival following release from fishing gear. We've identified a handful of relevant pubs and hope to identify a few more.

17 September 2020 3,194 6 View

How to study the effect of emerging contaminants in fish community?

Using marine organisms ( in particular demersal fish) I would like to study the effect of emerging contaminants and multiple stressor in these communities. Do you have any advice/recommendation?

04 June 2020 646 1 View

Small sample size in master thesis: What suggestions do you have how to statistically treat the data?

Dear everyone who might be able to help: Hello! I'm currently a student in marine science and I am writing my master thesis about Elasmobranchs and the big numbers that are being caught by...

19 February 2020 2,741 12 View

Small sample size in master thesis: What suggestions do you have how to statistically treat the data?

Dear everyone who might be able to help: Hello! I'm currently a student in marine science and I am writing my master thesis about Elasmobranchs and the big numbers that are being caught by...

01 February 2020 4,343 9 View

Finding plant gene from N-terminal sequence?

We have isolated a 30 KDa protein from a plant that hasn't been sequenced. We have 15 amino acids from N terminal Edman degradation. We are having trouble fishing the gene out. We have been...

11 December 2019 7,343 1 View

How to identify a DNA-binding protein by its binding DNA sequence?

I have a list of DNA sequences which I assume are bound by proteins. Now, I would like to identify the proteins that bind to these DNA sequences. I imagine this should be (theoretically) possible...

19 February 2019 8,027 3 View

Literature on electricity to control Lepomis sp.?

I am looking for literature listing voltages, pulse frequencies and cycles a) used to electro fish for Lepomis sp. (preferable Lepomis gibbosus) b) applied in electric fences that hold Lepomis sp....

06 July 2018 7,783 1 View