Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Why my Total RNA samples do not have clear two bands?

This is gel electrophoresis result for total RNA samples with good conc. However, I guess there is a problem with the purity of the samples, i can't figure out why it appears like this? And how...

22 February 2021 338 9 View

Can I make mixture of cinnamic acid with morin flavanoid ?

I am working on a project for assessing the Antibacterial activity of various chemicals, I was wondering Can i make solution of those components using water as a solvent, depending on the fact...

19 November 2020 7,857 4 View

Did anyone used microflow imaging to measure the size distribution of Multivesicular liposome which are charcterized with size (>3um)??

I have prepared Multivesicular liposomes using the following procedures: Multivesicular liposome formulations for the sustained deliv... Multivesicular liposome (MVL) sustained delivery of a...

18 November 2020 3,189 2 View

How antibodies differentiate according to match every epitope?

How antibodies differentiate according to match every epitope , ie: how takes its shape as a monoclonal

10 October 2020 9,573 2 View

I have 4 latent variables and each of which has 7 observed variables in SEM, how this affects the parsimony of the model?

I am conducting an investigation using SEM (Structural Equation Modelling) and I have 4 latent variables and each of which has 7 observed variables. How this affects the parsimony of the model?...

07 September 2020 718 3 View

How to express Nano-fluid in conservation equation of Mass, Momentum and Energy?

How to express Nano-fluid in conservation equation of Mass, Momentum and Energy?

07 September 2020 5,903 1 View

How to examine hypothesis in SmartPLS?

Should I report the Path coefficient or Latent variable correlations after bootstrapping as the P value and T-Statistics in the latent variable correlations of two hypotheses are met while in path...

07 September 2020 6,994 2 View

I followed a common procedure to synthesize Fe3O4 nanoparticles. The problem is that XRD pattern does not show any crystal structure. Could you help?

Besides, I have performed different characterization tecniques to my Fe3O4 nanoparticles and all are similar to what is reported in literature. Those characterization techniques include FTIR, SAED...

22 July 2020 8,549 12 View

How much water does each desalination plant type produce and cost?

There are many types of water desalination plants; Reverse Osmosis, MSF, Solar desalination units, ........... etc. How much water does each desalination plant type produce and what is the...

30 May 2020 7,719 4 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

I have a problem with kanamycin - why the kanamycin doesn't affect?

Hi everyone, I need to expression of GFP with pET28 plasmid in E. coli BL21(DE3) plysE. Transformed cells have kanamycin and chloramphenicol resistance. Normally, only transformed cells should...

22 February 2021 4,032 4 View

How does elongation affect the resistance of an electrode?

What are the testing rate for electrodes recommended by ASTM for cochlear implants. I am currently doing the 15mm/min elongatio testing.

22 February 2021 1,515 1 View

Resistivity of PVD TiNx thin films vs. N2 flow?

In the PVD of TiN thin films (~100A), I've found the resistivity of the TiN films dropped from ~100 to 60um*cm with N2 flow increasing up to the metallic mode ending point; then the resistivity...

21 February 2021 1,864 2 View

Where can the fault diagnosis data set of lithium battery be found?

Most of the data sets on the network only contain good information about the voltage and internal resistance of lithium batteries. The data of faulty battery is missing. Who can help me, I will be...

21 February 2021 8,659 1 View