Hello everyone,
I am currently designing a study for ion channel regulation.. and I wanted to know what are the protocols that are out there for the inhibition of Na+/K+ ATPase.
I have read few papers where people have used various concentration of NaCl,Hepes,glucose,MgCl2, CaCl2 to make potassium free media with various concentrations of Ouabain? Is there specific media that could be purchase and used? How essential are all these components to be added? Are there any good and most reliable protocol out there. Every paper I have read had different concentrations of reagents besides the fact they were studying variation of Ouabain.
Any help, papers, suggestion would be greatly appreciated.
Ouabain is the best known inhibitor of Na, K- ATPase. Check literature and you will get plenty of references.
Ouabain kill retinas at nanomolar concentrations, by contrast it do not kill muscle apparently. Crotamine did not killed retinas and it is deadly for muscle cells. It will depend on which cells you are talking about
he Kinetics of Non-Synaptically Triggered Acute Excitotoxic Responses in the Central Nervous System Observed Using Intrinsic Optical Signals
- April 2011
- CNS & neurological disorders drug targets 11(2):132-41
- DOI:10.2174/187152712800269704
Interaction of Small Cationic Peptides with Intact Basement Membranes. A Study Using Intrinsic Optical Signals of Chick Retinas
- March 2014
- Current Medicinal Chemistry 21(12):1458-1466
- DOI
- 10.2174/092986732112140319102212
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