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Questions related from Sana Nasim
Has anyone done FISH on cre-recombinased mice? I want to know if anyone has successfully been able to quench the autofluorescence from the those mice to successfully visualize the targeted genes?...
08 January 2020 3,746 3 View
I am looking for a protocol (cheap and effective way) that I could use to isolate multiple types of cells and be able to sort them out. For instance, I want to isolate multiple sub populations of...
30 July 2018 2,230 3 View
Has anyone done LCMS on condition media for protein quantification? I have this media (after the exposure of different flow conditions) that i would like to use LCMS for protein quantification...
13 February 2018 2,657 6 View
Hello everyone, I am currently designing a study for ion channel regulation.. and I wanted to know what are the protocols that are out there for the inhibition of Na+/K+ ATPase. I have read few...
26 November 2017 8,875 2 View
Has anyone used pig targeted gene expression using MiSeq (Illumina)? I have been looking into the literature but no success.. There are definitely pre-made library kits for Human, Mouse and...
22 June 2017 2,566 1 View
Hello, I want to look into MiSeq technique for gene expression analysis. I would like to know if there are any tips, guidelines or suggestions. Also if there are other ways to choose a...
20 June 2017 2,402 3 View
Hi, I have recently been trying to run RT-qPCR experiments for interstitial cells. I used Qiagen RNA extraction kit where I got ~500ng/ul of RNA with 260/280~2 and 260/230~1.9, which shows me that...
16 January 2017 9,790 11 View
Does anyone know of a method/tip to avoid interstitial cell contamination from endothelial cells? I have been trying to isolate valvular endothelial cells (pig) and keep getting interstitial cell...
08 December 2016 208 3 View
Does anyone has any software (free) recommendation for digitizing images? I have few echocardiogram images (velocity vs. time) that I would like to digitize. I have been trying to use...
28 November 2016 865 3 View
Hi, Does anyone know of a database for all the genes present at the pig's aortic valve? I am wondering if there is a database for that. I was able to find all the genes expressed in the pig but...
03 October 2016 262 0 View
Hello, Does anyone know of methods (preferably using Fiji) to assess quantitative data to analyze double positive cells. So I have figures from FITC/TRITC/DAPI channels and not all of my signals...
01 January 1970 2,481 2 View
