Sana Nasim

9 Questions 6 Answers 0 Followers

Questions related from Sana Nasim

In situ hybridization on cre-recombinased mice protocol?

Has anyone done FISH on cre-recombinased mice? I want to know if anyone has successfully been able to quench the autofluorescence from the those mice to successfully visualize the targeted genes?...

08 January 2020 3,703 3 View

Any recommended protocols for isolating multiple types of cells from a tissue sample?

I am looking for a protocol (cheap and effective way) that I could use to isolate multiple types of cells and be able to sort them out. For instance, I want to isolate multiple sub populations of...

30 July 2018 2,181 3 View

Liquid chromatography mass spectrometry (LC-MS) and condition media analysis?

Has anyone done LCMS on condition media for protein quantification? I have this media (after the exposure of different flow conditions) that i would like to use LCMS for protein quantification...

13 February 2018 2,589 6 View

Pig gene expression using MiSeq

Has anyone used pig targeted gene expression using MiSeq (Illumina)? I have been looking into the literature but no success.. There are definitely pre-made library kits for Human, Mouse and...

22 June 2017 2,513 1 View

Tips on MiSeq

Hello, I want to look into MiSeq technique for gene expression analysis. I would like to know if there are any tips, guidelines or suggestions. Also if there are other ways to choose a...

20 June 2017 2,349 3 View

How to perform cDNA and primer quality assessments?

Hi, I have recently been trying to run RT-qPCR experiments for interstitial cells. I used Qiagen RNA extraction kit where I got ~500ng/ul of RNA with 260/280~2 and 260/230~1.9, which shows me that...

16 January 2017 9,746 11 View

How to avoid interstitial cell contamination from endothelial cells?

Does anyone know of a method/tip to avoid interstitial cell contamination from endothelial cells? I have been trying to isolate valvular endothelial cells (pig) and keep getting interstitial cell...

08 December 2016 162 3 View

Any software recommendations for digitizing images?

Does anyone has any software (free) recommendation for digitizing images? I have few echocardiogram images (velocity vs. time) that I would like to digitize. I have been trying to use...

28 November 2016 832 3 View

Quantification of double positive signals from whole mount tissue in Fiji?

Hello, Does anyone know of methods (preferably using Fiji) to assess quantitative data to analyze double positive cells. So I have figures from FITC/TRITC/DAPI channels and not all of my signals...

01 January 1970 2,444 2 View