My rat golgi stained slices are falling apart during slicing in cryostat (-13°C is the best temperature for me to obtain at least some whole slices) and during color development specially after incubation with sodium thiosulfate solution. At the time I see the slices in the microscope are very brittle.
I'm trying to standardize Das et al 2013 protocol (The Golgi-cox method), in the laboratory, but I'm having some troubles specially because I can't get useful slices.
I agree with Dick. Use a vibratome because the blocks are to firm to cut them on a cryostat. In recent times a celoidine-embedding was recommended. Then you can cut this blocks on a microtom which you use for paraffin-sectioning but istaed of c or d- knifes you have use an a or b-knife. I guess this is not very commen because today most of the labs use disposible knife blades.
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