spread ur diluted sample on agar plate(2/3), observe them for isolated anabaena colony...or take diluted sample n pick up anabaena cells under inverted microscope with pasteur pipette

Dear Aqsa,

If the specific strain you are working with forms long filaments, you could try performing serial dilutions of your cultures and then selectively filtering your samples with membranes that allow solely Chlorella to pass through. Finally, the membranes should be thoroughly rinsed with culture medium and cultivated again.

In case the filaments are short or too sensitive to the shearing induced by filtration, I would suggest you to develop a very dilute culture and pick out the Chorella cells using a micro pipette and an inverted microscope, starting a new culture from that drop you have placed on the slide.

Regards,

Erico

Thank you Vikas U. Kapase and Érico de Godois Baroni for your suggestion. I have performed serial dilution method but could not get rid of chlorella. But now I will go for plate streaking method and membrane filtration. Thank you