How have professors been able to transition complex ideas and concepts to a virtual environment?

As COVID-19 has forced academia to react instantly to the dynamics of teaching and learning, I am curious to find out how you have been able to transfer complex ideas that you would normally teach...

17 May 2020 8,254 16 View

Can I activate a mixed primary (mouse) glial culture, and then shake microglia off?

Our lab usually uses the shaking method to isolate primary mouse microglia from mixed glial cultures. Till now, I have been collecting microglia, plating them in isolation, and then activating...

17 November 2019 2,048 1 View

What can be an alternative method for determining ECSA of Pt on a carbon paper electrode coated with 10% Pt/C?

I have tried H2 UPD and Cu UPD to no avail. The voltammograms do not show the required peaks on the desorption side to calculate the ECSA. The signal is quite weak to discern over the double layer...

02 August 2018 1,029 4 View

Has anyone had issues with extracting RNA from serum-grown bacterial cells?

In a side-by-side experiment, I got a normal amount of RNA from cells grown in chelexed BHI but very low yields from serum-grown cells. There was more cells in the serum culture, so low cell...

10 May 2018 2,167 3 View

What is the best molecular marker to separate healthy and pathological astrocytes?

I have quantified astrocytes with GFAP in a series of westerns. I would like to know if the lower level of GFAP I am seeing is because it is non-reactive due to a healthy environment or...

06 September 2017 5,783 1 View

Do you have experience or protocols for cryofixation and sectioning of fetal/embryonic pigs?

14 December 2016 2,639 4 View

I have a mixed-design (2: condition X 2: pre-post) but due to a problem with data collection am unable to match pre-test with post-test data. Help?

I am in a bit of a bind with a project evaluating a classroom-based intervention. The design was supposed to be a mixed-design whereby I collected pre-post data from willing students linking...

05 December 2016 938 4 View

Hi, does anyone have an idea on how to purify a molecule with free thiol function without risking its oxidation?

I am interested in getting pure molecules with free thiol but the problem is that during the synthesis I always get a mixture of free thiols and disulphide bridge. After the purification on...

24 May 2016 1,699 1 View

Does anyone have any information regarding a tool to measure attitudes and beliefs of clinical nurses regarding a specific clinical intervention?

Does anyone have any information regarding a tool to measure attitudes and beliefs of clinical nurses regarding a specific clinical intervention?

14 February 2016 5,014 3 View

How long should I fix in Bouins for Masson's Trichrome?

02 August 2015 3,718 11 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

How to write a scientific report?

We are analysing scientific reports at University in great detail, so I wanted to find a scientific report about how to write a scientific report. Including detail such as what information to put...

02 March 2021 7,602 4 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

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01 March 2021 3,905 5 View

How to Balance Bench work with Reading and Writing?

I am a second-year grad student. I am finding it difficult finding time to read literature and write (things like qualifying exam, thesis, grants). I find myself so busy with bench work that I...

01 March 2021 3,325 4 View

Which software is better to write systematic review paper?

We have seen some authors use some software to write review paper. As obvious, the study of all published papers concerning our studying field is not possible and these software can help us to...

27 February 2021 6,296 1 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View

What are the important tips for writing review articles?

Important tips for writing a good review articles

24 February 2021 8,544 6 View