Mutation detection by primers can be achieved using various techniques, such as PCR-based methods. Here's an overview of a commonly used PCR-based method called PCR amplification and DNA sequencing:
PCR-based methods, including PCR amplification and DNA sequencing, are widely used for detecting mutations in a specific DNA region. However, it's important to note that the specific technique chosen for mutation detection may depend on factors such as the size of the target region, the type of mutation being investigated, and the available resources and expertise in the laboratory. Other methods like allele-specific PCR, restriction fragment length polymorphism (RFLP) analysis, or high-resolution melting analysis (HRMA) can also be used for mutation detection, depending on the specific requirements of the study.
It is recommended to consult relevant literature, protocols, or experts in the field for specific guidance on mutation detection techniques based on your research needs and resources.
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