How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Orbitrap Fusion FT keep getting disconnected?

Hi, I been having a problem with the Orbitrap Fusion for quite a while and it has been getting more and more frequent, which is very frusttrating. The fusion FT would get disconnected suddenly...

22 January 2021 1,235 4 View

How can I differentiate the true allele from stutters and PCR artifacts in microsatellite genotyping?

Most of my microsatellite markers are dinucleotides, therefore they show stuttering. In regular stuttering is easy to identify the allele, which is the highest peak on the right, after the shorter...

26 September 2020 1,486 5 View

Is microsatellite study is useful for phylogenetic study of cyanobacteria?

Which is one is better in between 16S rRNA and microsatellite phylogentic study?

28 July 2020 1,633 2 View

How to interpret the Sentinel 2 image name?

Landsat 8 image name provides details of the date and path number, etc. Can anyone please provide similar interpretation of Sentinel 2 MSI details from this name? I can see the date, But path/...

27 July 2020 6,612 2 View

How do I determine if my Fst values are significantly different from 0 or between populations that are and are not separated by a highway?

I am trying to determine if a highway is too much of a barrier for shrews to cross and breed freely. What I have to work with is ~1080 bp of the mitochondrial cytochrome b gene (we did not do...

22 June 2020 7,647 1 View

Is it correct to compare Fst values obtained from different genetic markers?

Fst is used in many genetic studies which involve different markers (mtDNA, microsatellites, SNPs...) as methodologies and next-generation genetics improve. I was wondering if it would be correct...

27 April 2020 7,175 3 View

Why are microsatellite stutter peaks changing on repeated fragment analysis runs of same PCR product?

I've run a couple hundred samples using a microsatellite panel, and recently some of the runs show very exaggerated stutter peaks smaller than the true allele size. To troubleshoot the issue,...

01 April 2020 4,501 7 View

What are the pros and cons of ethanol vs phenol-chloroform extraction for DNA for microsatellites?

Previously for genotyping from mouse tissue, I have used an extraction method which involved SDS-proteinase k, followed by RNAse A and Isopropanol, then resuspension of remaining pellet in buffer...

01 February 2020 8,714 3 View

Dealing with next-gen SSR Microsatellite data from target enrichment experiment?

Hi All, we have a bioinformatics challenge and we would love any help this community can offer. We have data from a target-enrichment experiment that was supposed to capture certain...

09 January 2020 930 3 View

It is possible to do landscape genetics with sequence based data?

I have read that sequence based data are not powerful for analyze isolation by distance between population. However, I want to know if is possible to analyze the effect of environmental variables...

03 January 2020 9,797 5 View