I am working with female Balb/C strain mouse and I noticed some strangeness in them. Inıtially there is no problem about them. Now they don't have any mystacial vibrissae! Does someone have any experience like that!
Hi, I am currently trying to isolate immune cells from mouse colon tissue for my project. For this purpose, I am using a protocol which includes ; - Surgical removal of the colon tissue from...
22 October 2023 2,158 1 View
Greetings! I am working on a research project that includes adolescent stressful life events as one of the variables. As an instrument to measure this construct, I found the Life Events Checklist...
20 October 2020 8,023 5 View
Why is the concept of statistical convergence necessary? Is there anyone who can explain?
10 January 2020 6,337 6 View
I started work with Dream taq polymerase and its buffer but I have primer dimers. For solving this problem I tried gradient PCR from 60 to 66 °C. Is this problem from my primers or my agent?...
02 February 2016 533 5 View
I want to detect nitric oxide (NO) with flow cytometry. I found two agent which are 4,5-Diaminofluorescein diacetate solution and 5,6-Diaminofluorescein diacetate- Sigma-Aldrich. Which is the...
30 September 2015 8,525 8 View
I need this information ASAP!
22 January 2015 5,146 2 View
I have a table of contact angles for a given polymer with different surface modifications and another table for average cell spread areas (um^2) for each given surface. Is Pearson's product-moment...
22 August 2014 3,951 7 View
Can anyone recommend a good starting book for differential geometry? I want this book to be a book with geometric explanations and geometric interpretations.
01 January 1970 3,180 9 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View