Can we use RT PCR to asses inflammasome inhibitory activity ?

Currently, I am studying about the inflammasome activity inhibition using a compound in cultured microglial cell lines. As the common procedure to analyze inflammasome activity is by measuring the...

08 October 2018 1,179 4 View

Preparation stock solution of MGITC (malachite green isothiocyanate)?

21 March 2018 2,525 3 View

Which library is better for constructing deep reinforcement learning?

I know some popular libraries among researcher focusing on deep reinforcement learning such as TensorFlow, Theano, Caffe, Torch and CNTK. I usually work on both CPU and GPU. I don't need much...

22 May 2017 5,534 7 View

Which PHP framework do you think is the best for web development?

CodeIgniter, Yii, Laravel, Zend, Prado, CakePHP, Simfony

22 July 2013 7,194 7 View

There are some formula to determinde Coherence Time (Tc), which one is best suite for VANET ?

some researcher define Tc = 1/4fd, also Tc = 1/Bd (doppler spread), also Tc = 1/fd (doppler frekuency, Tc = 9/16fd, also Tc = 0,423/fd

01 January 1970 7,124 4 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

How do I change g/l into mM for bacto agar. I am trying to get the millimolar content of sodium and chloride percentage in bacto agar. How do I do it?

I am doing a salt tolerance-related experiment and for that I need to relate my salt levels to that in bacto agar. I don't want my salt concentrations to increase due to already salt content...

09 February 2021 4,158 1 View

Transformation into the Stbl3 chemo competent cells?

03 February 2021 1,111 5 View

Why do high doses of humic substances inhibit the growth of corn roots? Is there a toxicity effect?

Length, volume, surface area and dry mass of corn roots treated with humic substances increased up to 13 liters per hectare and decreased with higher doses.

02 February 2021 2,190 4 View

How to Remove DNA From a Sample Without Rupturing Yeast?

I transformed a plasmid into yeast and selected for transformants by growing them in a selective media (liquid, not agar plate). My experiment requires that I continually grow/ split these...

01 February 2021 9,936 2 View

How to store Fusarium oxysporum for long period in simple methods ?

31 January 2021 1,832 17 View

What is the composition of the solid and broth agar to grow Streptomyces from freezed sample? what are extraction steps of products from broth agar?

Please what are the composition of the solid agar could I use to grow the bacteria? what are the composition of the broth agar could I use to grow the bacteria and how to extract the natural...

26 January 2021 5,006 1 View