Monitoring reaction contain n-butyllithium?

25 October 2024 0 4K Report

I have performed reaction that involved alkylation of thiophene in presence of n-butyllithium but because I was not monitor the reaction I got a high percentage of unreacted alkylhalide. my question is can I monitor it by TLC while it under nitrogen atmosphere?

More Aisha Rr's questions See All

Hi researchers, has anybody ever used mouse somatostatin ELISA kit from novus biological Nbp2 -80274?

I have tried to use this kit to measure released somatostatin from isolated mouse islets, but I had an issue where all measurements were low which should not be the case. doe any body have any...

24 June 2024 1,552 0 View

Consent question for respondents for tenant satisfaction survey?

If any one did include any consent questions (few lines) in a tenant satisfaction survey. For example, we use de identified data from different housing providers tenants for benchmarking purposes....

19 May 2024 724 0 View

I need to know if anyone has a simple method to extract vitamin D from Oil?

There is a lot of matrix effect that disable me to quantify it. what i need is a quick and fast extraction method. what should i add?

13 May 2024 6,902 2 View

Hi Researchers, could anybody provide me with a protocol to prepare a cryosection for isolated mouse pancreatic islets ?

How to prepare the sections and how to immunostain with three different antibodies? As I am going to use antibody to stain receptor of interest and anti insulin and antiglucagon. How can the three...

28 December 2023 7,154 0 View

Hi researchers? How can I immunostain mouse pancreatic islets after isolatio?

Hi , I am looking for a full protocol for immunostaining of mouse pancreatic islets after isolation. I put them in RMPI after isolation. How can I prepare them for staining? and can I do...

05 October 2023 9,326 2 View

Portable water quality assessment of some selected sachet waters pdf?

portable water quality assessment of some selected sachet waters pdf?

22 September 2023 1,744 2 View

Radioligand competition assay?

I am going to do a radioligand competition binding assay, could any one help me with a protocol. The Kd value of my radioligand is 6.4. And the final volume of the assay is 500microliter, do I...

22 August 2023 2,236 0 View

Which approach or innovative technology is more effective for science education in the twenty-first century?

Which method or modern technology, as a science instructor, will help raise the academic performance of students in secondary school? An innovative approach to teaching of abstract concepts...

20 July 2023 1,715 8 View

35s GTP gamma s binding assay?

what is the cause of high scintillation counts in 35s GTP gamma s binding assay, despite of optimizing ligand concentration and all other parameters.

27 June 2023 1,234 0 View

Preparing sample for western blotting from pancreatic islets?

How can preparation of sample for western blotting from isolated pancreatic islet be done?

23 June 2023 7,871 0 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

How can I measure the thickness of thin film by Uv-Vis?

06 August 2024 7,810 3 View

Why is TLC not a form of partition chromatography?

Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?

03 August 2024 7,035 3 View

Is there any Indian Company or Startups, from any universiy or IITS, which manufacture Gas Chromatography instrument , i want to make a custOmized G?

or we can collaborate

01 August 2024 6,705 2 View

Is there a good freeware software for photodocumentation of TLC?

I need something like Camag's VisionCat software for photodocumentation of TLCs. Could you recommend something?

31 July 2024 8,415 1 View

How to improve peaks separation in HPLC?

Hi everyone, I'd like to better separate two close peaks coming from a bioconversion sample, I'm currently using a C18 KromaPhase 4,6mm Ø.I. SCHARLAU, particle size (µm): 10, pore size (Å): 100,...

30 July 2024 435 3 View

Gas chromatography RT detection?

I am working on algal extract to which gas chromatography (Not GC-MS) spectrum I want to discover. My question is can we identify specific compounds using retention time if I compared the RT with...

29 July 2024 8,034 4 View