Matpower for 3-phase unbalanced systems?

Hello, I have been using Matpower for several distribution studies, considering balanced loading conditions. Consequently, a single-phase simulation can be done. However, I am now working towards...

03 May 2020 4,218 3 View

Does somebody use Genalex for AFLP?

Hello everyone, I would like to count the PCoA in Genlaex for my AFLP matrix (it is from GeneMarker, binary- 0/1). I have 672 samples, 55 populations, with different number of samples in...

03 December 2018 4,575 3 View

Energy&Product Development bachelor?

Post to be removed

29 July 2018 4,627 1 View

Can you recommend really good anti-Rac1 antibody for immunofluorescence?

Dear Colleagues, I need specific anti-Rac1 antibody for immunofluorescence staining. I've tried two up to know (Cytoskeleton and Thermo), but the staining is predominantly nuclear or some...

20 February 2018 4,626 3 View

How to solve problem with sodium/lithium volatiliy in annealing?

Hello. I am dealing with sodium and lithium compounds, however it seems that a lot of sodium/lithium is evaporating during annealing process. Can you share your experience, how do you deal with...

11 December 2017 287 6 View

Strange vesicles in hTERT human astrocytes culture

Dear Researchers, I have a problem with immortalized human astrocytes. I did the immortalization on my own using lentivirus with hTERT (commercial). I performed the selection with puromycin and...

31 July 2017 4,148 4 View

How can I isolate RNA from primary embryonic neurons?

I need to isolate RNA from primary embryonic cortical neurons at different time points. I bought the cells from Life Technologies and plated them in a 48-well plate according to the company's...

28 January 2016 5,115 2 View

Is there any commercially available hyperlipemic cell culture serum?

06 January 2016 6,023 2 View

How do I re-select stable cell lines?

I transfected U251 cells, selected them with blastacidin (20 ug/ml) for 2 weeks and check the expression of the product by qPCR and western. Although the mRNA levels are quite good, I have very...

28 July 2015 5,535 4 View

How do I remove Propyl Parahydroxybenzoate and Methyl Parahydroxybenzoate from the sample with Fluocinolone acetonide ?

I have got HPLC method for identification related impurities and degradation products of fluocinolone acetonide, but two peaks (from degradation) are in the same place as Methyl...

22 March 2015 1,064 2 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

Timeout error!! Processor in the loop (PIL) using STM32F4 Matlab 2018a?

Dear Researchers I am trying to perform a PIL simulation using STM32F4 Discovery board and comunication serial USB TO TTL. During simulation I receive the following timeout error: An error...

01 March 2021 2,327 1 View

Is there any expert in Smart PLS who can help me?

Dear colleagues, I have a manuscript that needs an expert in smart PLS to run the analysis, Is there anyone expert and ready to help me, please?

01 March 2021 8,212 3 View